Journal: bioRxiv

Article Title: Acute and Lifelong Exercise Modulate the Tumorigenic Potential of Human Lung Cancer Cells and Their Susceptibility to Cisplatin

doi: 10.64898/2026.03.19.713009

Figure Lengend Snippet: Serum conditioning by acute and lifelong exercise had opposite effects on the proliferation rate of A549 human lung cancer cells. ( A ) For most of the participants in both cohorts, conditioning by acute exercise reduced sera’s ability to stimulate cell proliferation. ( B ) On average, the impact of the acute exercise intervention on serás ability to stimulate cell proliferation did not differ significantly (ns) between the two cohorts (right panel). ( C ) On average, cells grown in medium supplemented with baseline masters’ serum exhibited higher proliferation rates than those grown in medium supplemented with baseline non-exercisers’ serum. All cultures were established and grown in medium supplemented with 10% (v/v) human serum. The number of cells in culture at 24 h and 48 h post-seeding, used to calculate proliferation rates, was estimated using the sulforhodamine B (SRB) assay. Dots and squares represent individual values for the different sera and are the means of three independent experiments. In each of these experiments, all conditions (sera and time points) were tested in three replicate cultures. Each set of connected dots represents sera collected from the same participant before (left; Baseline) and after (right; Post-intervention) the acute exercise intervention. Large horizontal bars and associated smaller horizontal (error) bars represent means ± standard deviation for the 13 participants of each cohort. The statistical significance of the effect of lifelong exercise was assessed using Student’s unpaired t -test. All other comparisons were made by repeated measures two-way ANOVA, assuming sphericity. Whenever the null hypothesis was rejected, Šídák’s multiple comparison test was performed. Statistical significance was set at P < 0.05 and statistically significant differences between the indicated groups are shown by *, for P ˂ 0.05, and by **, for P < 0.01.

Article Snippet: Briefly, at each of the two time points, cells were fixed using a cold 10% (w/v) trichloroacetic acid (Merck; T6399) solution and were then stained using a 0.05% (w/v) SRB solution (Merck; 230162).

Techniques: Sulforhodamine B Assay, Standard Deviation, Comparison

Journal: bioRxiv

Article Title: Acute and Lifelong Exercise Modulate the Tumorigenic Potential of Human Lung Cancer Cells and Their Susceptibility to Cisplatin

doi: 10.64898/2026.03.19.713009

Figure Lengend Snippet: Lifelong exercise more than doubled the sensitivity of A549 human lung cancer cells to cisplatin. ( A ) Concentration-response curves depicting the cytotoxicity of cisplatin against cells stimulated with pooled sera (10% (v/v)) from non-exercisers and master athletes, obtained at rest (Baseline) and after an acute exercise intervention (Post-intervention). ( B ) CC 50 values for cisplatin for cultures incubated with the four different pooled sera. Cell viability was estimated at 72 h post-cisplatin addition using the sulforhodamine B (SRB) assay. For each pooled serum, the impact of the different cisplatin concentrations (0–455 μM) on cell viability was tested in three independent experiments, each with three replicate cultures per concentration, and expressed as percentage of the control value (0 µM cisplatin). CC 50 values were calculated from concentration-response curves generated using a nonlinear regression model in GraphPad Prism. Dots/diamonds and corresponding error bars represent, respectively, means and standard deviation for the three independent experiments. Comparisons between the indicated groups were made by both ( A ) the sum-of-squares using the extra sum-of-squares F test; ( B ) Student’s unpaired t-test (effect of lifelong exercise) and repeated measures two-way ANOVA, assuming sphericity (effect of acute exercise). Whenever the null hypothesis was rejected, Šídák’s multiple comparison test was performed. Statistical significance was set at P < 0.05 and statistically significant differences between the indicated groups are shown by *, for P ˂ 0.05, and by **, for P < 0.01. CC 50 , Half-maximal cytotoxic concentration.

Article Snippet: Briefly, at each of the two time points, cells were fixed using a cold 10% (w/v) trichloroacetic acid (Merck; T6399) solution and were then stained using a 0.05% (w/v) SRB solution (Merck; 230162).

Techniques: Concentration Assay, Incubation, Sulforhodamine B Assay, Control, Generated, Standard Deviation, Comparison

Journal: Genes & Diseases

Article Title: TIGAR promotes osteogenic differentiation and ameliorates glucocorticoid-induced osteoporosis via autophagy-Nrf2-ROS axis

doi: 10.1016/j.gendis.2025.101735

Figure Lengend Snippet: Effects of dexamethasone (Dex) on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and TIGAR expression. (A) BMSCs were treated with Dex for 24, 48, and 72 h, and cells viability was analyzed with sulforhodamine B (SRB) cytotoxicity assay kit. (B–D) BMSCs were treated with different concentration of Dex for 48 h, and mRNA levels of runt-related transcription factor 2 (Runx2) , Sp7 transcription factor/osterix ( Sp7) , and alkaline phosphatase ( Alpl) were detected with qRT-PCR. (E–G) Western blotting analysis and quantification of Runx2 and Sp7 in BMSCs treated with different concentration of Dex for 48 h. (H) Alkaline phosphatase (ALP) staining and quantification of ALP-positive cells showed osteoblast activity in BMSCs treated with Dex on Day 9. (I) Alizarin Red S staining and quantification of mineralized nodules showed the role of Dex on osteogenic differentiation on Day 14. (J–L) BMSCs were treated with different concentration of Dex for 48 h; then the expression of TIGAR was detected by qRT-PCR (J) and western blotting (K and L). (M, N) Western blot analysis and quantification of TIGAR in osteogenic differentiation at different times (0, 3, 6, 9, and 12 d after addition of osteogenic differentiation medium). Data are shown as mean ± SEM. n = 3, biologically independent samples. One-way analysis of variance (ANOVA) with Tukey's multiple comparisons test was used to assess statistical significance. ∗ p < 0.05, ∗∗ p < 0.01.

Article Snippet: Cell viability of BMSCs after Dex treatment was detected by sulforhodamine B (SRB) cytotoxicity assay kit (Yeasen Biotechnology Co., Ltd., Shanghai, China, 40205ES76).

Techniques: Expressing, Cytotoxicity Assay, Concentration Assay, Quantitative RT-PCR, Western Blot, Staining, Activity Assay